DNA/RNA Studies

  
        

A. Reagents for hybridyzation

Viagene provides non-radioactive kits for DNA/RNA hybridization. These kits do not include probes. For making hybridization probes, Viagene Biotech provides DNA/RNA Biotin labeling kits (PCR-, Random- or End-labeling). Viagene also offers molecular biology services (check) at competitive prices.

Catalog# Products Description Pack Price$
MBSB001a CSouth10 For Non-Radioactive Southern Hybridization 10/kit 376
NRBN001b CSouth05 For Non-Radioactive Southern Hybridization 5/Kit 215
NRBN013a CNorth10 Non-Radioactive Northern Hybridization 10/kit 397
NRBN013b CNorth5 Non-Radioactive Northern Hybridization 5/kit 235
NRBN003 DNA-Blk Non-Radioactive Southern Blocking Solution 66mL 55
NRBN004 DNA-Mbr Non-Radioactive Trans-blot membranes 4/box 38
NRBN005 HRP-INd Streptavidin-HRP buffer for DNA 5 blots 50
NRBN006 StrpHRPd Streptavidin-HRP for DNA 100ul 89
NRBN007 DNA-Wsh Washing Sol. for Hybridization 66ml 45
NRBN009 RNA-Blk Non-Radioactive Northern Blocking Solution 100ml 75
NRBN010 RNA-Mbr Non-Radioactive Trans-blot Membranes 1/bag 55
NRBN011a HRPINr10 Streptavidin-HRP Buffer for RNA 10 blots 65
NRBN011b HRPINr05 Streptavidin-HRP Buffer for RNA 5 blots 65
NRBN012 StrpHRPr Streptavidin-HRP, Northern Hybridization 100ul 65
NRBN013 RNAwSHr Non-Radioactive Northern Wash Solution 55ml 55
NRBN014 RNAequR Equilibrant Buffer, Non-Radioactive Northern 66ml 35
NRBN015 B-Marker Biotin-labeled DNA Marker 20 loadings 89
NRBN016 DLb-PCR DNA PCR Biotin-labeling 5 reaction 89
NRBN017 DLb_Nick DNA Nick-labeling, Biotin 5 reaction 89
NRBN018 DLb-Rndm DNA Random-label, Biotin 5 reaction 99
NRBN019 DLB-End DNA End-label with Biotin 5 reactions 89
SERV008 DothbrSV Dot-Bot Hybridization Customer Service 96 samples contact
SERV009 BprobeSV Biotin-Probe Preparation 200 reactions contact
SERV010 SouthSV1 Southern Hybridization Customer Service 20 samples contact
SERV011 SouthSV2 Southern Hybridization Customer Service 10 samples contact
SERV012 NorthSV1 Northern Hybridization Customer Service 20 samples contact

B. Reagents for DNA/RNA gel electrophoreses

Catalog# Products Pack Price$ Description
DCSR200 CoolStar 200 200ul 90 Non-EthBr DNA/RNA dyes
DCSR500 CoolStar 500 500ul 89 Non-EthBr DNA/RNA dyes

Non-ethidium bromide DNA/RNA staining dyes (Cat# DDY101, DCSR50Non EthBr0 & DCSR200)

Viagene Biotech has developed the nucleic acid gel stains , CoolBlue and CoolStar, for DNA-gel staining and Gel-stain EMSA. These stains are new-generation fluorescent nucleic acid gel stains designed to replace the highly mutagenic and carcinogenic ethidium bromide (EthBr). These CoolStarTM dyes are superior to EthBr by virtue of having a combination of high sensitivity, low toxicity, and great stability.

EthBr has been widely used for nucleic acid gel staining becauseNon-EthBr Dyes of its low cost and generally sufficient sensitivity. However, EthBr is highly toxic. The safety hazard and costs associated with decontamination and waste disposal can ultimately make the dye expensive and inconvenient to use.

◊ High Sensitivity.
◊ No Band-shift when prestaining DNA is used.
◊ Safer than EB. Shown by Ames test and other tests to be non-mutagenic and non-cytotoxic.
◊ Stable at room temperature.
◊ Compatible with standard blue LED and UV transilluminators.
◊ Use with blue light reduces mutagenesis for gel excision and subsequent cloning.
◊ No damage to RNA/DNA sample.

     

C. Instruments & Devices

Catalog# Products Description Pack Price$
IMGR002 CoolImager II Chemiluminescence & fluorescence Imager set 14,500
IMGR003 CoolImager III Chemiluminescence & fluorescence Imager set 18,200
BIBX001 CoolBlue480 LED Transilluminator(480nm) set 399
BIBX002 Cool-UV300 LED Transilluminator (300nM) set 569
BIBX003 CoolUV400 LED Transilluminator (400nm) set 569
BIBX004 CoolRed620 LED Transilluminator (620nm) set 499
BIBX005 Cool-IR680 LED Transilluminator (680nm) set 599
BIPS001 Dual power Dual Power (1501A) for electrophoresis set 499
BIGE001 Mini gel device Protein electrophoresis device II set 250
BIGE002 Mini Shaker Used for Immunoassays, Hybridization, or EMSA set 220

 

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