Instruments
I. Price of ECL iagers and substrate
Catalog#
Products
Description
Pack
Price.$
IMGR002
CoolImager-I
Chemiluminescence &
fluorescence Imager, type I
set
14500
IMGR003
CoolImager-II
Chemiluminescence &
fluorescence Imager, type II
set
18200
CHHL100
LightGen-HL
ECL substrat, high
sensitivity (detail)
100ml
198
CHHL050
LightGen-HL
ECL for imagers, high
sensitivity.
50ml
110
CHHL020
LightGen-HL
ECL for imagers, high
sensitivity.
20ml
55
CHCL01L
LightGen-CL
ECL for imagers, high
sensitivity.
1000ml
365
CHCL500
LightGen-CL
ECL for x-ray filmes (detail)
500ml
199
CHCL100
LightGen-CL
ECL for x-ray filmes (detail)
100ml
82
CHBL01L
LightGen-BL
ECL, liquid-based detection (detail)
1000ml
390
CHBL500
LightGen-BL
ECL, liquid-based detection (detail)
500ml
212
CHBL100
LightGen-BL
ECL, liquid-based detection (detail)
100ml
89
II. Chemiluminescent & Fluorescent Imager (IMGR002, IMGR003)
For the price of Cool Imager II
and III, click here..
For a list of
chemiluminescence-related products, click here.
For the
User Manual of Cool Imager II and III, click here.
Chemiluminescent and fluorescent imaging is used broadly for the study of proteins and DNA/RNA. Conventional imagers for imaging DNA gels cannot be used for detecting chemiluminescent signals due to their limited sensitivity. In order to detect chemiluminescent signals, CCD cameras must be cooled to a low temperature to reduce "Hot Spots". Cool ImagerTM - a chemiluminescent & fluorescent imaging workstation, developed by Viagene Biotech - is a compact, single integrated unit with state-of-the-art computer, microelectronics and semiconductor technology. It provides the capacity for rapid, high sensitivity capture of chemiluminescent and fluorescent images, which can be analyzed through pre-installed sophisticated “Cool Imaging" software. The combination of the Cool Imager’s high-integration and the deep-cooled CCD camera with focus-stabilized optics makes it possibly to achieve much better levels of sensitivity, dynamic range, linearity and resolution compared to other much more expensive imagers on the market. With Cool ImagerTM, all images are digital and one can completely eliminate the use of x-ray films and film developers for chemiluminescent imaging.
1. Applications:
Southern/Northern blots
Western blots
Low-density gene or
protein chip image
Agarose gels stained with EthBr or SYBR
Green.
Acrylamide gels stained with Coomassie Blue, SYPRO
Red, SYPRO Orange, and silver stain.
Quantification of dot
blots
Microplate assays, e.g. reporter gene assay.
Non-radioactive EMSA (Gel Shift Assays)
2. Features: See the feature page.
III. ECL Substrates
For prices of Chemiluminescence Substrates, click here.
For the User Manual of Lightgene™ HL, click here
For the User Manual of Lightgene™ HL, click here
1. Chemiluminescence Substrate (CAT#: CHHL100, CHCL01L & CHBL01L)
VViagene carries a variety of chemiluminescence Horseradish Peroxidase (HRP) substrates with different sensitivities for use in different types of experiments. These substrate products are suitable for chemiluminescent imaging of Western blotting analysis, Northern or Southern hybridization, and non-radioactive Electrophoretic Mobility Shift Assay (EMSA). They are all identified with the trademark of Lightgen™.
Features:
High Sensitivity
Duration and Durability
Competitive Price
2. Lightgen™HL (Cat#: CHHL100, -050, -020)
LLightgen™ HL is a chemiluminescence substrate with extremely high sensitivity for detecting Horseradish Peroxidase (HRP) on nitrocellulose, PVDF, or nylon membranes, suitable for use with digital imagers. It is used for Western blotting, Northern/Southern hybridization & Electrophoretic Mobility Shift Assay (EMSA). Lightgen™ HL can detect femtogram quantities of HRP. When used with X-ray films, even extremely weak signals can be detected, and a surprise result not previously detected may be obtained. For Western blotting, the amounts of investigators’ samples and antibodies used in the reactions may be reduced by 1/3 to 1/5 to obtain best results and save money on these reagents. When used for hybridization or EMSA, Lightgen™ HL provides much higher sensitivity than that obtained using radioisotope-labeled materials.
Features:
High Sensitivity
Duration and Durability
Competitive Price
Lightgen™ CL is a chemiluminescence substrate of Horseradish Peroxidase with medium sensitivity, suitable to be used for Western blotting, DNA/RNA hybridization and non-radioactive EMSA using detection by X-ray exposure. The reaction of Lightgen™ CL & HRP emits a blue light, which lasts for several hours. Lightgen™ CL is easy to use by combining equal amounts of Solution A and B. It has high sensitivity and can detect nanogram quantities of HRP on nitrocellulose, PVDF or nylon membrane.
Lightgen™ BL is a chemiluminescence substrate of Horseradish Peroxidase, optimized for liquid-based detections, such as chemiluminescent ELISA. It has high sensitivity and duration, and wide linear range of sample concentration, good for quantitative detection, and very suitable for immunoassays.
Features:
High Sensitivity
Duration and Durability
Competitive Price
