ECL Substrates

  
        

Catalog# Products Pack Price $ Description
CHBL01L LightGen-BL 1000ml 390 ECL, liquid-based detection (detail)
CHBL100 LightGen-BL 100ml 89 ECL, liquid-based detection (detail)
CHBL500 LightGen-BL 500ml 212 ECL, liquid-based detection (detail)
CHCL01L LightGen-CL 1000ml 365 ECL, medium sensitivity (detail)
CHCL100 LightGen-CL 100ml 82 ECL, medium sensitivity (detail)
CHCL500 LightGen-CL 500ml 199 ECL, medium sensitivity (detail)
CHHL020 LightGen-HL 20ml 55 ECL substrat, high sensitivity (detail)
CHHL050 LightGen-HL 50ml 110 ECL substrat, high sensitivity (detail)
CHHL100 LightGen-HL 100ml 198 ECL substrat, high sensitivity (detail)

For prices of Chemiluminescence Substrates, click here.

For the User Manual of Lightgene™ HL, click here

For the User Manual of Lightgene™ HL, click here

A. Chemiluminescence Substrate (CAT#: CHHL100, CHCL01L & CHBL01L)

Viagene carries a variety of chemiluminescence Horseradish Peroxidase (HRP) substrates with different sensitivities for use in different types of experiments. These substrate products are suitable for chemiluminescent imaging of Western blotting analysis, Northern or Southern hybridization, and non-radioactive Electrophoretic Mobility Shift Assay (EMSA). They are all identified with the trademark of Lightgen™.

Features:

High Sensitivity
Duration and Durability
Competitive Price

B. Lightgen™HL (Cat#: CHHL100, -050, -020)

Lightgen™ HL is a chemiluminescence substrate with extremely high sensitivity for detecting Horseradish Peroxidase (HRP) on nitrocellulose, PVDF, or nylon membranes, suitable for use with digital imagers. It is used for Western blotting, Northern/Southern hybridization & Electrophoretic Mobility Shift Assay (EMSA). Lightgen™ HL can detect femtogram quantities of HRP. When used with X-ray films, even extremely weak signals can be detected, and a surprise result not previously detected may be obtained. For Western blotting, the amounts of investigators’ samples and antibodies used in the reactions may be reduced by 1/3 to 1/5 to obtain best results and save money on these reagents. When used for hybridization or EMSA, Lightgen™ HL provides much higher sensitivity than that obtained using radioisotope-labeled materials.

Features:

High Sensitivity
Duration and Durability
Competitive Price

C. Lightgen™CL & BL (Cat#: CHCL01L, CHBL01L)

Lightgen™ CL is a chemiluminescence substrate of Horseradish Peroxidase with medium sensitivity, suitable to be used for Western blotting, DNA/RNA hybridization and non-radioactive EMSA using detection by X-ray exposure. The reaction of Lightgen™ CL & HRP emits a blue light, which lasts for several hours. Lightgen™ CL is easy to use by combining equal amounts of Solution A and B. It has high sensitivity and can detect nanogram quantities of HRP on nitrocellulose, PVDF or nylon membrane.

Lightgen™ BL is a chemiluminescence substrate of Horseradish Peroxidase, optimized for liquid-based detections, such as chemiluminescent ELISA. It has high sensitivity and duration, and wide linear range of sample concentration, good for quantitative detection, and very suitable for immunoassays.

Features:

High Sensitivity
Duration and Durability
Competitive Price

 

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