RNA EMSA

  
        
RNA EMSA样图

In recent years, evidence has emerged for the existence of many diverse types of RNA, which play roles in a wide range of biological processes in all kingdoms of life. These molecules generally do not, however, act in isolation, and identifying which proteins partner with RNA is a major challenge. Many methods, in vivo and in vitro, have been used to address this question, including combinatorial or high-throughput approaches, such as systematic evolution of ligands, cross-linking and immunoprecipitation and RNA immunoprecipitation combined with deep sequencing. However, most of these methods are not trivial to pursue and often require substantial optimization before results can be achieved. Here, we demonstrate a simple technique that allows one to screen proteins for RNA-binding properties in a gel-shift experiment and can be easily implemented in any laboratory. This assay should be a useful first-pass tool for assessing whether a protein has RNA- or DNA-binding properties, prior to committing resources to more complex procedures.

The principle of RNA EMSA as well as DNA EMSA is simple, considerations for performing a successful RNA EMA including the follows:

1)Needs instruments and reagents for RNA EMSA.

2) The knowledge for handling RNAs and proteins.

3) The skills for running a vertical gel electrophoresis.

4) The facility for imaging ECL signals via x-ray films or imagers for imaging ECL or infrared fluorescence.

5) The RNA probe is expensive, 20 fold of what for making a DNA probes.

6) Most probes for RNA EMSA is a single-strand RNA, which is very fragile for degradation.

7) Working environment should keep as RNase-free.

8) Based on the considerations above, we recommend our IRFluo rEMSA kits for RNA EMSA for its fast results (~2 hours) and simple operation (imaging gels immediately after gel electrophoresis).

9) If using Viagene's RNA EMSA service, the results would be secured as the scientists in Viagene Biotech have extensive experience for RNA EMSA (Linking to technique services).

Related Products

Catalog No/Products Description Pack Price($)
IMGPR003/CoolImager III High resolution Fluorescence & Chemiluminescence Imager set 18200
SIDET111a/CoolShift Non-radioactive EMSA kit w/o probes & trans-membranes. 100 assays 395
SIDET111b/CoolShift Non-radioactive EMSA kit w/o probes & trans-membranes. 50 assays 295
SERV005a/EMSA-PN Nuclear protein isolation kit for EMSA
 50 isolations 95
SERV005b/EMSA-PC EMSA Positive Control 5 loadings 75
SERV005C/EMSA-NC EMSA Negative Control 5 loadings 55
TFRGT105/Poly[dI:dC] A Reagent, Reducing Non-specific Binding 50 assays 49
TFRGT108/EMSA-Memb Non-radioactive EMSA Transfer Membrane 4 sheets 35
TFRGT104/EMSA-RBuf EMSA Probe/Reaction Buffers 50 assays 20
TFRGT110/ESA-HRPbuf Streptavidin HRP Incubation Buffer 50 assays 50
TFRGT103a/EMSA_BBuf Non-radioactive EMSA, Blocking Solution 60ml/bottle 60
TFRGT103b/EMSA_WBuf Non-radioactive EMSA, Washing Sol. 60ml/bottle 40
TFRGT103c/EMSA_EBuf Non-radioactive EMSA, Equilibration Sol. 66ml/bottle 20
SIDET037a/EMSABio50 Non-radioactive EMSA, Biotin-Probe only 50 assays 85
SIDET037b/EMSABioP100 Non-radioactive EMSA, Biotin-Probe 100 assays 120
SERVOO6/EMSA_SCP EMSA Specific Competition Probe 25μL 70
SERV007/EMSA_SSH EMSA Super Shift (Customer provides Abs) each 50
SINP002/EMSA_TNPX Tissue Non-denatured Nuclear Protein Extract 50 samples 95
SERVO16/EMSA_vTNP Service for Tissue Nuclear Protein Extract 80+20*samp. *
SERV017/BEMSA_vCNP Service for cell nuclear protein extract 80+15*samp *
SERV018/EMSA_vbBP Custom Synthesis of Bio-Probe for EMSA 1 pair 136
SINP005/EMSA_ProC Kit for determining nuclear protein Concent. 100mL/bottle 78
SERV004/EMSA_vNPC Customer Service for Nuclear Protein Concentration 65+5* sample *
SINP012/EMSA_preC Pre-treatment for mammalian cells for EMSA 50 samples 120
       
       
       
       

  

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